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Comparison of the effect of bisphenols on parental and differentiated H9c2 cells
Macáková, Barbora ; Jirkovský, Eduard (advisor) ; Macháček, Miloslav (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Barbora Macáková Supervisor: PharmDr. Eduard Jirkovský, Ph.D. Title of diploma thesis: Comparison of the effect of bisphenols on parental and differentiated H9c2 cells Bisphenol is compound largely found in products and environment around us. Its toxicity and danger for human health is still being studied, meanwhile direct influence of environmentally relevant doses was not discovered. These endocrine disruptors are affecting variousreceptorsin bodiesand effecton cardiovascularsystem isnotexcludedeither.The most extensively studied substance is BPA, which is gradually being replaced by its analogs, although these are not extensively researched to the same extent. In this work we focused on influence of bisphenol BP, M and S on differentiation of H9c2 cells, serving as a model with potential negative effects on heart development and its regeneration after damage. H9c2 cell line has an undifferentiated phenotype but can be easily directed towards differentiation under certain conditions Research was focused on impact of bisphenol on differentiated cells and its changes in the expression of selected differentiation markers (GATA4, Hand2, Tnnt2, Myog) (transcription factors). Set protocols were...
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Characterization of different differentiation protocols of H9c2 cardiomyoblasts
Gardianová, Anna ; Jirkovský, Eduard (advisor) ; Macháček, Miloslav (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Anna Gardianová Supervisor: PharmDr. Eduard Jirkovský, Ph.D. Title of diploma thesis: Characterization of different differentiation protocols of H9c2 cardiomyoblasts Cell differentiation is a common process in the development of multicellular organisms leading to an alteration of cell's appearance and function. The process of differentiation plays a significant role in the reparation of damaged tissues too. Some chemicals may have negative effects on these processes and therefore affect human health. This thesis focuses on characterization of differentiation processes of H9c2 cell line using various published differentiation protocols, and to describe changes over time in the morphology (occurrence of multinucleated and elongated cells) and mRNA expression of selected markers typical for the cardiac (cTnT, Hand2, GATA4) or skeletal (myogenin) muscle phenotype. We monitored the process of the differentiation for 15 days and the individual protocols differed by the addition of fetal bovine serum (FBS, 1 % or 10 %) and all-trans- retinoic acid (ATRA, 10 nmol/L) to the growth medium Dulbecco's Modified Eagle's Medium. From what we have observed, the results show that all of the utilized protocols...
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